Stem cell differentiation is a complex, multi-factorial biological process, which the Rothman lab seeks to illuminate. Via genomic screens in embryonic stem cells, Dr. Rothman's goal is to decipher the transcriptional network orchestrating the initial stages of differentiation, thus enabling controlled differentiation into distinctive lineages. Working in the Columbia Genome Center High-Throughput Facility, Rothman is able to sort through literally millions of combinations of genes and morphogenes which will allow the overall pattern of control to reveal itself. His program of high-throughput stem cell biology should deliver a comprehensive overview, opening new approaches for the field and new practical opportunities, as well as key foundations for developing unifying concepts.
Mayer T, Jagla B, Wyler MR, Kelly PD, Aulner N, Beard M, Barger G, Tobben U, Smith DH, Brandon L, Rothman JE. Cell-based assays using primary endothelial cells to study multiple steps in inflammation. Methods Enzymol. 2006;414:266-83.
Yamamoto A, Cremona ML, Rothman JE. Autophagy-mediated clearance of huntingtin aggregates triggered by the insulin-signaling pathway.
J Cell Biol. 2006 Feb 27;172(5):719-31.
Antinozzi PA, Garcia-Diaz A, Hu C, Rothman JE. Functional mapping of disease susceptibility loci using cell biology. Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3698-703. Epub 2006 Feb 28.