The NYU Fertility Center is currently pursuing two hESC projects with IRB approval. The first project is to generate novel disease models from genetically abnormal embryos as diagnosed by Preimplantation Genetic Diagnosis, focusing on fatal, prevalent, and incurable conditions such as Tay-Sachs and cystic fibrosis. Second, to generate novel types of hESC lines from individual cells of discarded frozen embryos which are most undifferentiated or lineage-allocated, allowing for the development of enhanced stem cell differentiation protocols and the study of totipotency, lineage allocation, and plasticity in human embryonic cells. Patients are currently being recruited and experimental conditions established by mouse ES cells.
Xu Y, Zhang JJ, Grifo JA, Krey LC. DNA methylation patterns in human tripronucleate zygotes. Mol Hum Reprod. 2005 Mar;11(3):167-71. Epub 2005 Feb 4.
Xu Y, Zhang JJ, Grifo JA, Krey LC. DNA methylation patterns in human tripronucleate zygotes. Mol Hum Reprod. 2005 Mar;11(3):167-71. Epub 2005 Feb 4.
Hansis C, Grifo JA, Krey LC. Candidate lineage marker genes in human preimplantation embryos. Reprod Biomed Online. 2004 May;8(5):577-83.