Professor Gronostajski's lab uses mouse Embryonic Stem Cells (mES) to make mice with mutations in the genes for the Nuclear Factor I transcription factors. They have cultured mES cells to make homozygous mutant cells from heterozygous cells, to delete genes in targeted mES cells using Cre-recombinase, and to create embryoid bodies to promote differentiation of mES cells into multiple cell lineages. Gronostajski's lab is culturing mouse neural stem cells (NSC) to assess changes in NSC number in some of our NFI knockout mice and investigating the effect of loss of NFI genes on the differentiation of NSCs into neural and glial cells.
Campbell CE, Piper M, Plachez C, Yeh YT, Baizer JS, Osinski JM, Litwack ED, Richards LJ, Gronostajski RM. The transcription factor Nfix is essential for normal brain development. BMC Dev Biol. 2008 May 13;8:52.
Plachez C, Lindwall C, Sunn N, Piper M, Moldrich RX, Campbell CE, Osinski JM, Gronostajski RM, Richards LJ. Nuclear factor I gene expression in the developing forebrain. J Comp Neurol. 2008 May 20;508(3):385-401.
Wang W, Mullikin-Kilpatrick D, Crandall JE, Gronostajski RM, Litwack ED, Kilpatrick DL. Nuclear factor I coordinates multiple phases of cerebellar granule cell development via regulation of cell adhesion molecules.
J Neurosci. 2007 Jun 6;27(23):6115-27.