Professor Sinha's laboratory is interested in understanding the molecular mechanisms that underlie the development of skin's epidermis and its appendages from multipotent stem cells. The Sinha lab studies how skin stem cells achieve remarkable ability to both self-renew and to commit to proliferate and differentiate. The lab applies a wide array of powerful molecular, genetic and biochemical techniques to study skin cells primarily in transgenic mouse models. The fate of skin cells is governed in part by transcription factors, which are specialized proteins that control the molecular destiny of a cell by turning genes on and off. The team has developed several mouse models where the levels of critical transcription factors can be altered in the skin epithelium in a controlled fashion. These models allow for study of the underlying mechanisms that control the homeostasis of the skin epithelium, and analysis of changes that lead to altered differentiation and development of skin diseases such as cancer.
Ortt K, Raveh E, Gat U, Sinha S. A chromatin immunoprecipitation screen in mouse keratinocytes reveals Runx1 as a direct transcriptional target of DeltaNp63.
J Cell Biochem. 2008 Jul 1;104(4):1204-19.
Nagarajan P, Romano RA, Sinha S. Transcriptional control of the differentiation program of interfollicular epidermal keratinocytes. Crit Rev Eukaryot Gene Expr. 2008;18(1):57-79. Review.
Nagarajan P, Sinha S. Development of an inducible gene expression system for primary murine keratinocytes. J Dermatol Sci. 2008 Jan;49(1):73-84. Epub 2007 Oct 25.