Muscular dystrophies are clinically and molecularly heterogenous diseases characterized by progressive wasting of skeletal muscle and lack of mobility. Dr. Bhagavati's lab is interested in developing cell-based therapies for these and other muscle disorders. Bhagavati is focused on identifying and isolating mouse skeletal muscle specific stem/progenitor cells by targeting reporter genes by homologous recombination to the earliest genes known to be expressed in developing skeletal muscle progenitors, such as Pax3, Pax7 and Myf 5. The lab is working to generate myogenic stem cells, from developing ES cells, by cell-cell fusion studies. Myogenic stem cells isolated in this way will be analyzed for their in vitro proliferative and differentiation characteristics, and potentially promising cell populations will be tested for their capacity to generate skeletal muscle after transplantation into dystrophic mice.
Bhagavati, S., Xu, W. (2005). Generation of skeletal muscle from transplanted embryonic stem cells in dystrophic mice. Biochem Biophys Res Commun. 2005 Jul 29;333(2):644-9.
Bhagavati, S., Xu, W. (2004). Isolation and enrichment of skeletal muscle progenitor cells from mouse bone marrow. Biochem Biophys Res Commun. 2004 May 21;318(1): 119-24.