The focus of Professor Andreadis's research is vascular tissue engineering, wound healing and lentiviral gene delivery. Specifically, we are using mesenchymal stem cells from bone marrow and hair follicle stem cells to engineer small diameter blood vessels for vascular tissue regeneration. We developed methods to purify smooth muscle progenitor cells and endothelial cells from bone marrow MSC and used them to engineer small diameter blood vessels. When implanted into the jugular vein of an ovine animal model our vessels remained patent for several weeks, suggesting that they have high potential for clinical use. In contrast to vessels engineered with cells from umbilical veins, those engineered with bone marrow derived cells, exhibited high elastogenic potential. More recently, my laboratory identified another source of stem cells, namely hair follicle stem cells, which may provide an easily accessible cell source for smooth muscle progenitor cells. We found that these cells can differentiate towards fat, bone and cartilage suggesting that the may resemble mesenchymal stem cells from bone marrow, thereby providing an easily accessible source of multipotent stem cells for tissue engineering and regenerative medicine.
Liu, J.Y., Peng, H.F. and Andreadis, S.T. (2008) Contractile smooth muscle cells derived from hair follicle stem cells. Cardiovasc. Res. 79(1): 24-33.
Liu, J.Y., Swartz, D.D., Peng, H.F., Gugino, S.F., Russell, J.A. and Andreadis, S.T. (2007). Functional tissue-engineered blood vessels from bone marrow progenitor cells. Cardiovasc. Res. 75(3): 618-628.
Bajaj, B.G., Lei, P. and Andreadis, S.T. (2005). Efficient gene transfer to human epidermal keratinocytes: in vitro evidence for transduction of epidermal stem cells. Mol. Ther. 11(6): 969-979.